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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20562-1-AP

Synonyms

EIEE4, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STXBP1 Polyclonal antibody (20562-1-AP)

    WB analysis using 20562-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20562-1-AP (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using STXBP1 Polyclonal antibody (20562-1-AP)

    IHC staining of human brain using 20562-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using STXBP1 Polyclonal antibody (20562-1-AP)

    IHC staining of human brain using 20562-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STXBP1 Polyclonal antibody (20562-1-AP)

    IF Staining of HeLa using 20562-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STXBP1 antibody (20562-1-AP) at dilution of 1:50 and CoraLite594-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20562-1-AP targets STXBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 1
Calculated molecular weight 68 kDa
Observed molecular weight68 kDa
GenBank accession numberNM_003165
Gene symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_10694824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions. The antibody is specific to STXBP1.

Protocols

Product Specific Protocols
WB protocol for STXBP1 antibody 20562-1-APDownload protocol
IHC protocol for STXBP1 antibody 20562-1-APDownload protocol
IF protocol for STXBP1 antibody 20562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols