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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STXBP2 Monoclonal antibody (66238-1-Ig)

    WB analysis using 66238-1-Ig (same clone as 66238-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    WB analysis of fetal human brain using 66238-1-Ig (same clone as 66238-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using STXBP2 Monoclonal antibody (66238-1-Ig)

    IP experiment of K-562 using 66238-1-Ig (same clone as 66238-1-PBS)

    IP result of anti-STXBP2 (IP:66238-1-Ig, 5ug; Detection:66238-1-Ig 1:2000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of human lung cancer using 66238-1-Ig (same clone as 66238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STXBP2 Monoclonal antibody (66238-1-Ig)

    IHC staining of human lung cancer using 66238-1-Ig (same clone as 66238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STXBP2 Monoclonal antibody (66238-1-Ig)

    IF Staining of HepG2 using 66238-1-Ig (same clone as 66238-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66238-1-Ig(STXBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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STXBP2 Monoclonal antibody, PBS Only

STXBP2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66238-1-PBS
Clone No.3G3A12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Protein unc-18 homolog 2, pp10122, Munc18-2, MUNC18 2, Hunc18b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66238-1-PBS targets STXBP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STXBP2 fusion protein Ag7640 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 2
Calculated molecular weight 593 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC002869
Gene Symbol STXBP2
Gene ID (NCBI) 6813
RRIDAB_2881627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15833
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.