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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using STXBP3 Polyclonal antibody (13764-1-AP)

    WB analysis of HepG2 using 13764-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using STXBP3 Polyclonal antibody (13764-1-AP)

    WB analysis of K-562 using 13764-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using STXBP3 Polyclonal antibody (13764-1-AP)

    IP experiment of K-562 using 13764-1-AP

    IP result of anti-STXBP3 (IP:13764-1-AP, 4ug; Detection:13764-1-AP 1:500) with K-562 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STXBP3 Polyclonal antibody (13764-1-AP)

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STXBP3 Polyclonal antibody (13764-1-AP)

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 40x lens).

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STXBP3 Polyclonal antibody

STXBP3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13764-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C, PSP, STXBP3, syntaxin binding protein 3, UNC 18C, Unc18 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

13764-1-AP targets STXBP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STXBP3 fusion protein Ag4747 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 3
Calculated molecular weight 592 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC038099
Gene Symbol STXBP3
Gene ID (NCBI) 6814
RRIDAB_2196832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00186
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STXBP3 antibody 13764-1-APDownload protocol
IHC protocol for STXBP3 antibody 13764-1-APDownload protocol
IP protocol for STXBP3 antibody 13764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Diabetes

PMID: 20460426

Authors - Pontus Boström
View Article
mouseWB

J Biol Chem

microRNA-15b contributes to depression-like behavior in mice by affecting synaptic protein levels and function in the nucleus accumbens.

Authors - Li Guo
View Article
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
View Article