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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STXBP4 Polyclonal antibody

STXBP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14231-1-AP

Synonyms

Syntaxin-binding protein 4, Syntaxin 4-interacting protein, Synip, STX4-interacting protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STXBP4 Polyclonal antibody (14231-1-AP)

    WB analysis using 14231-1-AP

    PC-3 lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using STXBP4 Polyclonal antibody (14231-1-AP)

    WB analysis of human heart using 14231-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using STXBP4 Polyclonal antibody (14231-1-AP)

    IHC staining of human pancreas cancer using 14231-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using STXBP4 Polyclonal antibody (14231-1-AP)

    IHC staining of human pancreas cancer using 14231-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using STXBP4 Polyclonal antibody (14231-1-AP)

    IF Staining of BxPC-3 using 14231-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using STXBP4 antibody (14231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inPC-3 cells, human heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14231-1-AP targets STXBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STXBP4 fusion protein Ag5471 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 4
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC041485
Gene symbol STXBP4
Gene ID (NCBI) 252983
RRIDAB_2196980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STXBP4 antibody 14231-1-APDownload protocol
IHC protocol for STXBP4 antibody 14231-1-APDownload protocol
IF protocol for STXBP4 antibody 14231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols