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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STXBP6 Polyclonal antibody

STXBP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10976-4-AP

Synonyms

Amisyn, Syntaxin-binding protein 6, STXBP 6, HSPC156

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using STXBP6 Polyclonal antibody (10976-4-AP)

    WB analysis of mouse brain using 10976-4-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STXBP6 Polyclonal antibody (10976-4-AP)

    WB analysis using 10976-4-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using STXBP6 Polyclonal antibody (10976-4-AP)

    WB analysis of human brain using 10976-4-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using STXBP6 Polyclonal antibody (10976-4-AP)

    WB analysis of human heart using 10976-4-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using STXBP6 Polyclonal antibody (10976-4-AP)

    WB analysis of human heart using 10976-4-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using STXBP6 Polyclonal antibody (10976-4-AP)

    WB analysis of BxPC-3 using 10976-4-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STXBP6 Polyclonal antibody (10976-4-AP)

    IHC staining of human lung cancer using 10976-4-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STXBP6 Polyclonal antibody (10976-4-AP)

    IHC staining of human lung cancer using 10976-4-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STXBP6 Polyclonal antibody (10976-4-AP)

    IHC staining of human lung cancer using 10976-4-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10976-4-AP (STXBP6 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, human brain tissue, BxPC-3 cells, rat brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10976-4-AP targets STXBP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STXBP6 fusion protein Ag1427 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 6 (amisyn)
Calculated molecular weight 25 kDa or 45 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC009499
Gene symbol STXBP6
Gene ID (NCBI) 29091
RRIDAB_2197002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STXBP6 antibody 10976-4-APDownload protocol
IHC protocol for STXBP6 antibody 10976-4-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB

BMC Med

A functional variant rs912304 for late-onset T1D risk contributes to islet dysfunction by regulating proinflammatory cytokine-responsive gene STXBP6 expression

Authors - Yu Qian
View Article