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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STYXL1 Polyclonal antibody (16433-1-AP)

    WB analysis using 16433-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16433-1-AP (STYXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STYXL1 Polyclonal antibody (16433-1-AP)

    WB analysis using 16433-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16433-1-AP (STYXL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STYXL1 Polyclonal antibody (16433-1-AP)

    IF Staining of HeLa using 16433-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STYXL1 antibody (16433-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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STYXL1 Polyclonal antibody

STYXL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16433-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MKSTYX, MK STYX, Map kinase phosphatase-like protein MK-STYX, Inactive dual specificity protein phosphatase MK-STYX, DUSP24

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCI-H1299 cells, PC-12 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16433-1-AP targets STYXL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STYXL1 fusion protein Ag9743 相同性解析による交差性が予測される生物種
Full Name serine/threonine/tyrosine interacting-like 1
Calculated molecular weight 313 aa, 36 kDa
Observed molecular weight25-36 kDa
GenBank accession numberBC024035
Gene Symbol STYXL1
Gene ID (NCBI) 51657
RRIDAB_3085490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6J8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STYXL1, Serine/threonine/tyrosine-interacting-like protein 1, is an inner mitochondrial membrane protein that plays a role in induction of stimulus-dependent apoptosis via the intrinsic apoptotic pathway (PMID: 21262771). STYXL1 catalyzes inactive phosphatase (PMID: 20180778, PMID: 23163895). By binding to G3BP1, STYXL1 inhibits the formation of G3BP1-induced stress granules (PMID: 20180778, PMID: 23163895).

Protocols

Product Specific Protocols
WB protocol for STYXL1 antibody 16433-1-APDownload protocol
IF protocol for STYXL1 antibody 16433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int J Biol Sci

LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.

Authors - Yan Fang
View Article
mouseWB

Int Immunopharmacol

Linderalactone mitigates diabetic cardiomyopathy in mice via suppressing the MAPK/ATF6 pathway

Authors - Xue Han
View Article