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KD/KO Validated

SUB1 Polyclonal antibody

Name: SUB1 Polyclonal antibody
Brand: Proteintech
SKU: 10948-2-AP

SUB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10948-2-AP

Synonyms

RPO2TC1, Positive cofactor 4, PC4, P15, p14

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of A549 using 10948-2-AP
WB result of SUB1 antibody (10948-2-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected A549 cells.
WB analysis of HeLa using 10948-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10948-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10948-2-AP
WB result of SUB1 antibody (10948-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected HeLa cells.
WB analysis of A549 using 10948-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10948-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10948-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 10948-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10948-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas cancer using 10948-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas cancer using 10948-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10948-2-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUB1 antibody (10948-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 10948-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A549 cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

10948-2-AP targets SUB1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SUB1 fusion protein Ag1390 相同性解析による交差性が予測される生物種
Full Name	SUB1 homolog (S. cerevisiae)
Calculated molecular weight	14 kDa
Observed molecular weight	18-23 kDa
GenBank accession number	BC009610
Gene symbol	SUB1
Gene ID (NCBI)	10923
RRID	AB_2255452
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#10948-2-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).

Protocols

Product Specific Protocols
WB protocol for SUB1 antibody 10948-2-AP	Download protocol
IHC protocol for SUB1 antibody 10948-2-AP	Download protocol
IF protocol for SUB1 antibody 10948-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	J Proteome Res Novel breast cancer metastasis-associated proteins. Authors - Ho Jiapei J View Article
mouse	WB	J Cell Biochem Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes. Authors - Li Lu L View Article
human,mouse	WB,IHC,IF,CoIP	Sci China Life Sci SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1 Authors - Hao Wang KO Validated View Article
human	WB	J Am Chem Soc HMGB3 and SUB1 Bind to and Facilitate the Repair of N2-Alkylguanine Lesions in DNA Authors - Ting Zhao KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SUB1 Polyclonal antibody

SUB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of A549 using 10948-2-AP

WB analysis of HeLa using 10948-2-AP

WB analysis using 10948-2-AP

WB analysis of HeLa using 10948-2-AP

WB analysis of A549 using 10948-2-AP

WB analysis of HeLa using 10948-2-AP

WB analysis of A549 using 10948-2-AP

IHC staining of human pancreas cancer using 10948-2-AP

IHC staining of human pancreas cancer using 10948-2-AP

IHC staining of human pancreas cancer using 10948-2-AP

IHC staining of human pancreas cancer using 10948-2-AP

IF Staining of HeLa using 10948-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Novel breast cancer metastasis-associated proteins.

Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes.

SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1

HMGB3 and SUB1 Bind to and Facilitate the Repair of N2-Alkylguanine Lesions in DNA