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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SUCLA2 Monoclonal antibody

SUCLA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C7E4

Cat no : 68549-1-Ig

Synonyms

A BETA, SCS betaA, SUCLA2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUCLA2 Monoclonal antibody (68549-1-Ig)

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SUCLA2 Monoclonal antibody (68549-1-Ig)

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SUCLA2 Monoclonal antibody (68549-1-Ig)

    IF Staining of Caco-2 using 68549-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68549-1-Ig targets SUCLA2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SUCLA2 fusion protein Ag17967 相同性解析による交差性が予測される生物種
Full Name succinate-CoA ligase, ADP-forming, beta subunit
Calculated molecular weight 463 aa, 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC027587
Gene symbol SUCLA2
Gene ID (NCBI) 8803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).

Protocols

Product Specific Protocols
WB protocol for SUCLA2 antibody 68549-1-IgDownload protocol
IF protocol for SUCLA2 antibody 68549-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols