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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUCLA2 Monoclonal antibody (68549-1-Ig)

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SUCLA2 Monoclonal antibody (68549-1-Ig)

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SUCLA2 Monoclonal antibody (68549-1-Ig)

    IHC staining of human colon cancer using 68549-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68549-1-Ig (SUCLA2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SUCLA2 Monoclonal antibody (68549-1-Ig)

    IF Staining of Caco-2 using 68549-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SUCLA2 Monoclonal antibody (68549-1-Ig)

    IF Staining of HepG2 using 68549-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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SUCLA2 Monoclonal antibody

SUCLA2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68549-1-Ig
Clone No.2C7E4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SCS betaA, EC:6.2.1.5, ATP-specific succinyl-CoA synthetase subunit beta, A-SCS, A BETA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68549-1-Ig targets SUCLA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SUCLA2 fusion protein Ag17967 相同性解析による交差性が予測される生物種
Full Name succinate-CoA ligase, ADP-forming, beta subunit
Calculated molecular weight 463 aa, 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC027587
Gene Symbol SUCLA2
Gene ID (NCBI) 8803
RRIDAB_3670383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P2R7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).

Protocols

Product Specific Protocols
WB protocol for SUCLA2 antibody 68549-1-IgDownload protocol
IHC protocol for SUCLA2 antibody 68549-1-IgDownload protocol
IF protocol for SUCLA2 antibody 68549-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols