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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SUCLG1 Polyclonal antibody, PBS Only

SUCLG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14923-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUCLG1 Polyclonal antibody (14923-1-AP)

    WB analysis using 14923-1-AP (same clone as 14923-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    WB analysis of rat liver using 14923-1-AP (same clone as 14923-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IP experiment of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    IP result of anti-SUCLG1 (IP:14923-1-AP, 4ug; Detection:14923-1-AP 1:500) with mouse kidney tissue lysate 2800 ug. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IHC staining of human liver cancer using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14923-1-AP (SUCLG1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14923-1-PBS targets SUCLG1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUCLG1 fusion protein Ag6481 相同性解析による交差性が予測される生物種
Full Name succinate-CoA ligase, alpha subunit
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC000504
Gene symbol SUCLG1
Gene ID (NCBI) 8802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).