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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUCLG2 Polyclonal antibody (14240-1-AP)

    WB analysis using 14240-1-AP (same clone as 14240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14240-1-AP (SUCLG2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using SUCLG2 Polyclonal antibody (14240-1-AP)

    IP experiment of mouse kidney using 14240-1-AP (same clone as 14240-1-PBS)

    IP result of anti-SUCLG2 (IP:14240-1-AP, 4ug; Detection:14240-1-AP 1:600) with mouse kidney tissue lysate 4800ug. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SUCLG2 Polyclonal antibody (14240-1-AP)

    IHC staining of human colon cancer using 14240-1-AP (same clone as 14240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14240-1-AP (SUCLG2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SUCLG2 Polyclonal antibody (14240-1-AP)

    IHC staining of human colon cancer using 14240-1-AP (same clone as 14240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14240-1-AP (SUCLG2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SUCLG2 Polyclonal antibody (14240-1-AP)

    IHC staining of human gliomas using 14240-1-AP (same clone as 14240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas slide using 14240-1-AP (SUCLG2 Antibody) at dilution of 1:25. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SUCLG2 Polyclonal antibody (14240-1-AP)

    IHC staining of human gliomas using 14240-1-AP (same clone as 14240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas slide using 14240-1-AP (SUCLG2 Antibody) at dilution of 1:25. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SUCLG2 Polyclonal antibody, PBS Only

SUCLG2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14240-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14240-1-PBS targets SUCLG2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUCLG2 fusion protein Ag5495 相同性解析による交差性が予測される生物種
Full Name succinate-CoA ligase, GDP-forming, beta subunit
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC047024
Gene Symbol SUCLG2
Gene ID (NCBI) 8801
RRIDAB_2197187
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96I99
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SUCLG2, encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. The deficiency of SUCLG2 represents an encephalomyopathic form of mtDNA depletion syndromes (PMID:30470562). Mutated SUCLG1 causes mislocalization of SUCLG2 protein, morphological alterations of mitochondria and an early-onset severe neurometabolic disorder. SUCLG2 is mainly expressed in liver, kidney, heart (PMID: 9765291).