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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SULF1 Polyclonal antibody

SULF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27438-1-AP

Synonyms

Extracellular sulfatase Sulf-2 secreted form, Extracellular sulfatase Sulf-1, Extracellular sulfatase Sulf 1, EC:3.1.6.14, EC:3.1.6.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SULF1 Polyclonal antibody (27438-1-AP)

    WB analysis using 27438-1-AP

    HT-1080 cell lysates were subjected to SDS PAGE followed by western blot with 27438-1-AP (SULF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SULF1 Polyclonal antibody (27438-1-AP)

    IHC staining of mouse skeletal muscle using 27438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27438-1-AP (SULF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SULF1 Polyclonal antibody (27438-1-AP)

    IHC staining of mouse skeletal muscle using 27438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27438-1-AP (SULF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHT-1080 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27438-1-AP targets SULF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SULF1 fusion protein Ag26758 相同性解析による交差性が予測される生物種
Full Name sulfatase 1
Observed molecular weight101 kDa
GenBank accession numberBC068565
Gene symbol SULF1
Gene ID (NCBI) 23213
RRIDAB_3085960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SULF1 antibody 27438-1-APDownload protocol
IHC protocol for SULF1 antibody 27438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols