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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SULT1B1 Polyclonal antibody (16050-1-AP)

    WB analysis using 16050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    WB analysis of human colon using 16050-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    WB analysis of human liver using 16050-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    IHC staining of human liver cancer using 16050-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    IHC staining of human colon cancer using 16050-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    IHC staining of human colon cancer using 16050-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:240 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SULT1B1 Polyclonal antibody

SULT1B1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16050-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SULT1B2, ST1B2, ST1B1, EC:2.8.2.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, human colon tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:120-1:480
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16050-1-AP targets SULT1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SULT1B1 fusion protein Ag9009 相同性解析による交差性が予測される生物種
Full Name sulfotransferase family, cytosolic, 1B, member 1
Calculated molecular weight 296 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC010895
Gene Symbol SULT1B1
Gene ID (NCBI) 27284
RRIDAB_2302767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43704
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).

Protocols

Product Specific Protocols
WB protocol for SULT1B1 antibody 16050-1-APDownload protocol
IHC protocol for SULT1B1 antibody 16050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols