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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SULT4A1 Monoclonal antibody

SULT4A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B9F6

Cat no : 66106-1-Ig

Synonyms

NST, hBR-STL-1, hBR-STL, EC:2.8.2.-, Brain sulfotransferase-like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using SULT4A1 Monoclonal antibody (66106-1-Ig)

    WB analysis of human brain using 66106-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66106-1-Ig (SULT4A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SULT4A1 Monoclonal antibody (66106-1-Ig)

    IHC staining of mouse brain using 66106-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66106-1-Ig (SULT4A1 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SULT4A1 Monoclonal antibody (66106-1-Ig)

    IHC staining of mouse brain using 66106-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66106-1-Ig (SULT4A1 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SULT4A1 Monoclonal antibody (66106-1-Ig)

    IF Staining of mouse brain using 66106-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SULT4A1 antibody (66106-1-Ig, Clone: 1B9F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SULT4A1 Monoclonal antibody (66106-1-Ig)

    IF Staining of mouse brain using 66106-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SULT4A1 antibody (66106-1-Ig, Clone: 1B9F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHuman brain, tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66106-1-Ig targets SULT4A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SULT4A1 fusion protein Ag17962 相同性解析による交差性が予測される生物種
Full Name sulfotransferase family 4A, member 1
Calculated molecular weight 284 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC022459
Gene symbol SULT4A1
Gene ID (NCBI) 25830
RRIDAB_2881505
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SULT4A1, also named as SULTX3, NST, ST4A1, hBR-STL and hBR-STL-1, belongs to the sulfotransferase 1 family. It may catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. SULT4A1 displays activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphtylamine, and 2-naphtol. It may have a role in the sulfation of drugs and neurotransmitters in the CNS. SULT4A1 is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. It is a novel cytosolic sulfotransferase that is primarily expressed in the brain.

Protocols

Product Specific Protocols
WB protocol for SULT4A1 antibody 66106-1-IgDownload protocol
IHC protocol for SULT4A1 antibody 66106-1-IgDownload protocol
IF protocol for SULT4A1 antibody 66106-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols