• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ChromoTek SUMO-Tag-Trap Magnetic Agarose

The ChromoTek SUMO-Tag-Trap Magnetic Agarose consists of an anti-SUMO-Tag Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of SUMO-tagged proteins from cell extracts of various organisms. The ChromoTek SUMO-Tag-Trap can also be used in conjunction with SUMO proteases such as SenP2 for on-bead digestion of SUMO-Tag fusion proteins to release the protein of interest.

Specificity

SUMO-Tag (all common variants)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Cat no : sutma

Synonyms

SUMO tag, SUMOStar, SUMO

Validation Data Gallery

  • Immunoprecipitation of three different variants of SUMO-tagged, GFP fusion proteins from E. coli cell lysates using SUMO-Tag-Trap Magnetic Agarose. WB analysis was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using ChromoTek Mouse anti-Spot-tag Antibody (28a5) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005).

    Immunoprecipitation of three different variants of SUMO-tagged, GFP fusion proteins from E. coli cell lysates using SUMO-Tag-Trap Magnetic Agarose. WB analysis was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using ChromoTek Mouse anti-Spot-tag Antibody (28a5) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005).

  • Immunoprecipitation of three different variants of SUMO-tagged, GFP fusion proteins from HEK293T cell lysates using SUMO-Tag-Trap Magnetic Agarose (sutma) followed by on-bead digest and elution with SenP2 protease. The majority of the cleaved protein is released after the 1st elution (E1), with the SenP2 and SUMO-tag remaining with beads in the residual (R) fraction. SUMOStar is resistant to SenP2 and was used as a control.

    Immunoprecipitation of three different variants of SUMO-tagged, GFP fusion proteins from HEK293T cell lysates using SUMO-Tag-Trap Magnetic Agarose (sutma) followed by on-bead digest and elution with SenP2 protease. The majority of the cleaved protein is released after the 1st elution (E1), with the SenP2 and SUMO-tag remaining with beads in the residual (R) fraction. SUMOStar is resistant to SenP2 and was used as a control.

Product Information

The ChromoTek SUMO-Tag-Trap Magnetic Agarose consists of an anti-SUMO-Tag Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of SUMO-tagged proteins from cell extracts of various organisms. The ChromoTek SUMO-Tag-Trap can also be used in conjunction with SUMO proteases such as SenP2 for on-bead digestion of SUMO-Tag fusion proteins to release the protein of interest.

DescriptionImmunoprecipitation of SUMO-tagged proteins with anti-SUMO-Tag Nanobody conjugated to magnetic agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, yeast, etc.

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to all common variants of the SUMO-Tag. The SUMO-Tag is based on Small Ubiquitin-like Modifier (SUMO) proteins of a size of ca. 11 kDa, which are covalently attached to target proteins as a post-translational modification. Fusion of the SUMO-Tag to a protein of interest (POI) may increase expression and solubility of the POI. Also, the SUMO-Tag can be specifically removed by SUMO proteases such as SenP2 without leaving non-native residues behind. At least three SUMO variants are commonly used as SUMO-Tag and are all recognized by the ChromoTek SUMO-Tag-Trap: the yeast SUMO homolog SMT3, the human SUMO3 and SUMOStar, a version of SMT3 resistant to SUMO proteases. Please note that the ChromoTek SUMO-Tag-Trap will also bind non-discriminatorily to endogenous SUMO homologs such as SUMO1, SUMO2 and SUMO3 present in human cells.
Binding Capacity30 μg of recombinant SUMO-tagged protein (~40 kDa) per 25 μL bead slurry
ConjugateMagnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
Wash Buffer Compatibility1 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.0% SDS, 2 M Urea
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity (KD)50 nM
Compatibility with mass spectrometryThe SUMO-Tag-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3668963
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!
Size25ul/reactions (eg：20rxns=500ul slurry)