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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUMO1 Polyclonal antibody (10329-1-AP)

    WB analysis using 10329-1-AP (same clone as 10329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10329-1-AP (SUMO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SUMO1 Polyclonal antibody (10329-1-AP)

    IP experiment of HeLa using 10329-1-AP (same clone as 10329-1-PBS)

    IP result of anti-SUMO1 (IP:10329-1-AP, 4ug; Detection:10329-1-AP 1:800) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SUMO1 Polyclonal antibody (10329-1-AP)

    IHC staining of human testis using 10329-1-AP (same clone as 10329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SUMO1 Polyclonal antibody (10329-1-AP)

    IHC staining of human testis using 10329-1-AP (same clone as 10329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SUMO1 Polyclonal antibody (10329-1-AP)

    IF Staining of A549 using 10329-1-AP (same clone as 10329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (10329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using SUMO1 Polyclonal antibody (10329-1-AP)

    FC experiment of A549 using 10329-1-AP (same clone as 10329-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human SUMO1 (10329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 10329-1-PBS in a different storage buffer formulation.

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SUMO1 Polyclonal antibody, PBS Only

SUMO1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 10329-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Small ubiquitin-related modifier 1, Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10329-1-PBS targets SUMO1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUMO1 fusion protein Ag0414 相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Calculated molecular weight 12 kDa
Observed molecular weight12~18 kDa, 80-90 kDa
GenBank accession numberBC006462
Gene Symbol SUMO1
Gene ID (NCBI) 7341
RRIDAB_2286872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP63165
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).