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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SUMO1 Monoclonal antibody, PBS Only

SUMO1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B5B5

Cat no : 67559-1-PBS

Synonyms

Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, GAP-modifying protein 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SUMO1 Monoclonal antibody (67559-1-Ig)

    WB analysis using 67559-1-Ig (same clone as 67559-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67559-1-Ig (SUMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SUMO1 Monoclonal antibody (67559-1-Ig)

    IHC staining of rat kidney using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using SUMO1 Monoclonal antibody (67559-1-Ig)

    IHC staining of rat small intestine using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SUMO1 Monoclonal antibody (67559-1-Ig)

    IHC staining of mouse kidney using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SUMO1 Monoclonal antibody (67559-1-Ig)

    IF Staining of MCF-7 using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SUMO1 Monoclonal antibody (67559-1-Ig)

    IF Staining of A549 using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:3200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67559-1-PBS targets SUMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SUMO1 fusion protein Ag29402 相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Calculated molecular weight 12 kDa
Observed molecular weight 15-100 kDa
GenBank accession numberBC006462
Gene symbol SUMO1
Gene ID (NCBI) 7341
RRIDAB_2882773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.This antibody can detect endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 80-90 kD).