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  • Featured Product
  • KD/KO Validated

SUMO2/3 Monoclonal antibody, PBS Only

SUMO2/3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1B3

Cat no : 67154-1-PBS

Synonyms

SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    WB analysis of HEK-293 using 67154-1-Ig (same clone as 67154-1-PBS)

    WB result of SUMO2/3 antibody (67154-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    WB analysis using 67154-1-Ig (same clone as 67154-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    WB analysis of A549 using 67154-1-Ig (same clone as 67154-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    WB analysis of NIH/3T3 using 67154-1-Ig (same clone as 67154-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    IHC staining of human breast cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    IHC staining of human breast cancer using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUMO2/3 Monoclonal antibody (67154-1-Ig)

    IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67154-1-PBS targets SUMO2/3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SUMO2/3 fusion protein Ag28672 相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Calculated molecular weight 11 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC008450
Gene symbol SUMO2
Gene ID (NCBI) 6613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.