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  • Featured Product
  • KD/KO Validated

SUMO2/3 Polyclonal antibody

SUMO2/3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10947-1-AP

Synonyms

SUMO2, SMT3B, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SUMO2/3 Polyclonal antibody (10947-1-AP)

    WB analysis of HEK-293 using 10947-1-AP

    WB result of SUMO2/3 antibody (10947-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using SUMO2/3 Polyclonal antibody (10947-1-AP)

    WB analysis of HEK-293 using 10947-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SUMO2/3 Polyclonal antibody (10947-1-AP)

    WB analysis of Jurkat using 10947-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SUMO2/3 Polyclonal antibody (10947-1-AP)

    WB analysis of A549 using 10947-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Recombinant protein using SUMO2/3 Polyclonal antibody (10947-1-AP)

    WB analysis of Recombinant protein using 10947-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SUMO2/3 Polyclonal antibody (10947-1-AP)

    IHC staining of human colon cancer using 10947-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10947-1-AP (SUMO2/3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SUMO2/3 Polyclonal antibody (10947-1-AP)

    IHC staining of human colon cancer using 10947-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10947-1-AP (SUMO2/3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUMO2/3 Polyclonal antibody (10947-1-AP)

    IF Staining of HeLa using 10947-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (10947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, A549 cells, Recombinant protein protein
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10947-1-AP targets SUMO2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUMO2/3 fusion protein Ag1388 相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Calculated molecular weight 11 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC008450
Gene symbol SUMO2
Gene ID (NCBI) 6613
RRIDAB_2198406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Protocols

Product Specific Protocols
WB protocol for SUMO2/3 antibody 10947-1-APDownload protocol
IHC protocol for SUMO2/3 antibody 10947-1-APDownload protocol
IF protocol for SUMO2/3 antibody 10947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Death Discov

Loss of PML nuclear bodies in familial amyotrophic lateral sclerosis-frontotemporal dementia

Authors - Francesco Antoniani
View Article