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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUMO2/3 Polyclonal antibody (10699-1-AP)

    WB analysis using 10699-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10699-1-AP (SUMO2/3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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SUMO2/3 Polyclonal antibody

SUMO2/3 Polyclonal Antibody for WB, ELISA
Cat No. 10699-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SUMO3, SUMO 3, SUMO 2, SMT3H1, SMT3A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10699-1-AP targets SUMO2/3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUMO2/3 fusion protein Ag1135 相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
Calculated molecular weight 12 kDa
Observed molecular weight 11-20 kDa
GenBank accession numberBC008420
Gene Symbol SUMO3
Gene ID (NCBI) 6612
RRIDAB_3669122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Protocols

Product Specific Protocols
WB protocol for SUMO2/3 antibody 10699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols