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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SUMO2/3 Recombinant antibody

SUMO2/3 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241156E1

Cat no : 84017-5-RR

Synonyms

SUMO3, SUMO 2, SMT3H1, SMT3A, SMT3 homolog 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SUMO3 Recombinant antibody (84017-5-RR)

    IF Staining of HepG2 using 84017-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SUMO3 Recombinant antibody (84017-5-RR)

    IF Staining of A431 using 84017-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SUMO2/3 Recombinant antibody (84017-5-RR)

    IF Staining of HepG2 using 84017-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO2/3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Affinity and Kinetic Characterization of 84017-5-RR

    Affinity and Kinetic Characterization of 84017-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84017-5-RR against Human SUMO2/3 were performed. The affinity constant is 6.81 nM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84017-5-RR targets SUMO2/3 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SUMO2/3 fusion protein Ag1135 相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
Calculated molecular weight 12 kDa
GenBank accession numberBC008420
Gene symbol SUMO3
Gene ID (NCBI) 6612
RRIDAB_3671586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Protocols

Product Specific Protocols
IF protocol for SUMO2/3 antibody 84017-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols