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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUN2 Polyclonal antibody (27556-1-AP)

    WB analysis using 27556-1-AP (same clone as 27556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SUN2 Polyclonal antibody (27556-1-AP)

    WB analysis of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

    WB result of SUN2 antibody (27556-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUN2 transfected HeLa cells. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SUN2 Polyclonal antibody (27556-1-AP)

    WB analysis of mouse testis using 27556-1-AP (same clone as 27556-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUN2 Polyclonal antibody (27556-1-AP)

    IF Staining of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUN2 antibody (27556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SUN2 Polyclonal antibody, PBS Only

SUN2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27556-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

UNC84B, SUN domain-containing protein 2, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27556-1-PBS targets SUN2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUN2 fusion protein Ag16077 相同性解析による交差性が予測される生物種
Full Name unc-84 homolog B (C. elegans)
Calculated molecular weight 717 aa, 80 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC111717
Gene Symbol SUN2
Gene ID (NCBI) 25777
RRIDAB_2880906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UH99
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).