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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUN2 Recombinant antibody (85103-3-RR)

    WB analysis using 85103-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SUN2 Recombinant antibody (85103-3-RR)

    WB analysis of HeLa using 85103-3-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEPG2 cells using SUN2 Recombinant antibody (85103-3-RR)

    IF Staining of HEPG2 using 85103-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUN2 antibody (85103-3-RR, Clone: 242533G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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SUN2 Recombinant antibody

SUN2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85103-3-RR
Clone No.242533G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

UNC84B, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B, KIAA0668

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Saos-2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85103-3-RR targets SUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SUN2 fusion protein Ag16077 相同性解析による交差性が予測される生物種
Full Name unc-84 homolog B (C. elegans)
Calculated molecular weight 717 aa, 80 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC111717
Gene Symbol SUN2
Gene ID (NCBI) 25777
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UH99
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).

Protocols

Product Specific Protocols
WB protocol for SUN2 antibody 85103-3-RRDownload protocol
IF protocol for SUN2 antibody 85103-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols