Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, Saos-2 cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85103-3-RR targets SUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SUN2 fusion protein Ag16077 相同性解析による交差性が予測される生物種 |
Full Name | unc-84 homolog B (C. elegans) |
Calculated molecular weight | 717 aa, 80 kDa |
Observed molecular weight | 80-85 kDa |
GenBank accession number | BC111717 |
Gene Symbol | SUN2 |
Gene ID (NCBI) | 25777 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UH99 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).
Protocols
Product Specific Protocols | |
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WB protocol for SUN2 antibody 85103-3-RR | Download protocol |
IF protocol for SUN2 antibody 85103-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |