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  • Featured Product
  • KD/KO Validated

SUPT16H Monoclonal antibody, PBS Only

SUPT16H Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4C4

Cat no : 68243-1-PBS

Synonyms

FACT140, FACT 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, CDC68

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUPT16H Monoclonal antibody (68243-1-Ig)

    WB analysis using 68243-1-Ig (same clone as 68243-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68243-1-Ig (SUPT16H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SUPT16H Monoclonal antibody (68243-1-Ig)

    WB analysis of MCF-7 using 68243-1-Ig (same clone as 68243-1-PBS)

    WB result of SUPT16H antibody (68243-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUPT16H transfected MCF-7 cells. This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SUPT16H Monoclonal antibody (68243-1-Ig)

    IHC staining of human colon using 68243-1-Ig (same clone as 68243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68243-1-Ig (SUPT16H antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUPT16H Monoclonal antibody (68243-1-Ig)

    IF Staining of HeLa using 68243-1-Ig (same clone as 68243-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPT16H antibody (68243-1-Ig, Clone: 1E4C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68243-1-PBS targets SUPT16H in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SUPT16H fusion protein Ag29459 相同性解析による交差性が予測される生物種
Full Name suppressor of Ty 16 homolog (S. cerevisiae)
Calculated molecular weight 120 kDa
Observed molecular weight135 kDa
GenBank accession numberNM_007192
Gene symbol SUPT16H
Gene ID (NCBI) 11198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SUPT16H, also named as FACT140, FACTP140, SPT16 and CDC68, belongs to the peptidase M24 family and SPT16 subfamily. SUPT16H is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). It also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.