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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SUPT6H Polyclonal antibody (23073-1-AP)

    WB analysis of HeLa using 23073-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SUPT6H Polyclonal antibody (23073-1-AP)

    WB analysis of HEK-293 using 23073-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SUPT6H Polyclonal antibody (23073-1-AP)

    WB analysis of Jurkat using 23073-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SUPT6H Polyclonal antibody (23073-1-AP)

    IP experiment of HEK-293 using 23073-1-AP

    IP result of anti-SUPT6H (IP:23073-1-AP, 4ug; Detection:23073-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SUPT6H Polyclonal antibody (23073-1-AP)

    IHC staining of human stomach cancer using 23073-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SUPT6H Polyclonal antibody

SUPT6H Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 23073-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

SPT6H, SPT6, KIAA0162, hSPT6, Histone chaperone suppressor of Ty6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23073-1-AP targets SUPT6H in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUPT6H fusion protein Ag19426 相同性解析による交差性が予測される生物種
Full Name suppressor of Ty 6 homolog (S. cerevisiae)
Calculated molecular weight 1726 aa, 199 kDa
Observed molecular weight 230 kDa
GenBank accession numberBC136524
Gene Symbol SUPT6H
Gene ID (NCBI) 6830
RRIDAB_11232593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7KZ85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and directly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

Protocols

Product Specific Protocols
WB protocol for SUPT6H antibody 23073-1-APDownload protocol
IHC protocol for SUPT6H antibody 23073-1-APDownload protocol
IP protocol for SUPT6H antibody 23073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article