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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SUPT7L Polyclonal antibody (25606-1-AP)

    WB analysis of HeLa using 25606-1-AP (same clone as 25606-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25606-1-AP (SUPT7L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of human brain using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of human brain using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of human gliomas using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of rat brain using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of rat brain using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of mouse brain using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of mouse brain using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SUPT7L Polyclonal antibody (25606-1-AP)

    IHC staining of human gliomas using 25606-1-AP (same clone as 25606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUPT7L Polyclonal antibody (25606-1-AP)

    IF Staining of HeLa using 25606-1-AP (same clone as 25606-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25606-1-AP (SUPT7L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25606-1-PBS in a different storage buffer formulation.

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SUPT7L Polyclonal antibody, PBS Only

SUPT7L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25606-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25606-1-PBS targets SUPT7L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUPT7L fusion protein Ag22264 相同性解析による交差性が予測される生物種
Full Name suppressor of Ty 7 (S. cerevisiae)-like
Calculated molecular weight 414 aa, 46 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC074000
Gene Symbol SUPT7L
Gene ID (NCBI) 9913
RRIDAB_2880156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94864
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SUPT7L, also named as KIAA0764, STAF65gamma, is a component of the STAGA transcription coactivator-HAT complex. The MW of SUPT7L is 46-65 kDa.