SURF1 Recombinant antibody
SURF1 Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
230210F11
Cat no : 83052-5-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83052-5-RR targets SURF1 in IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SURF1 fusion protein Ag3959 相同性解析による交差性が予測される生物種 |
Full Name | surfeit 1 |
Calculated molecular weight | 33 kDa |
Observed molecular weight | 30-31 kDa |
GenBank accession number | BC028314 |
Gene symbol | SURF1 |
Gene ID (NCBI) | 6834 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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IHC protocol for SURF1 antibody 83052-5-RR | Download protocol |
IF protocol for SURF1 antibody 83052-5-RR | Download protocol |
IP protocol for SURF1 antibody 83052-5-RR | Download protocol |
FC protocol for SURF1 antibody 83052-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |