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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SURF1 Recombinant antibody (83052-6-RR)

    WB analysis of MCF-7 using 83052-6-RR

    WB result of SURF1 antibody (83052-6-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SURF1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using SURF1 Recombinant antibody (83052-6-RR)

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SURF1 Recombinant antibody (83052-6-RR)

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using SURF1 Recombinant antibody (83052-6-RR)

    FC experiment of HepG2 using 83052-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83052-6-RR

    Affinity and Kinetic Characterization of 83052-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83052-6-RR against Human SURF1 were performed. The affinity constant is 0.509 nM.

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  • Featured Product
  • KD/KO Validated

SURF1 Recombinant antibody

SURF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83052-6-RR
Clone No.230210E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat heart tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83052-6-RR targets SURF1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SURF1 fusion protein Ag3959 相同性解析による交差性が予測される生物種
Full Name surfeit 1
Calculated molecular weight 33 kDa
Observed molecular weight30-31 kDa
GenBank accession numberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
RRIDAB_3670776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SURF1 antibody 83052-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols