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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

SURVIVIN Polyclonal antibody

SURVIVIN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10508-1-AP

Synonyms

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SURVIVIN Polyclonal antibody (10508-1-AP)

    WB analysis of Jurkat using 10508-1-AP

    WB result of Survivin antibody (10508-1-AP, 1:5000) with si-control and si-Survivin transfected Jurkat cells..

  • Western Blot (WB) analysis of various lysates using SURVIVIN Polyclonal antibody (10508-1-AP)

    WB analysis using 10508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SURVIVIN Polyclonal antibody (10508-1-AP)

    WB analysis of NIH/3T3 using 10508-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SURVIVIN Polyclonal antibody (10508-1-AP)

    WB analysis using 10508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SURVIVIN Polyclonal antibody (10508-1-AP)

    WB analysis of PC-3 using 10508-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of U2OS cells using SURVIVIN Polyclonal antibody (10508-1-AP)

    IP experiment of U2OS using 10508-1-AP

    IP result of anti-SURVIVIN (IP:10508-1-AP, 4ug; Detection:10508-1-AP 1:1000) with U2OS cells lysate 440 ug.

  • Immunoprecipitation (IP) experiment of Jurkat cells using SURVIVIN Polyclonal antibody (10508-1-AP)

    IP experiment of Jurkat using 10508-1-AP

    IP result of anti-SURVIVIN (IP:10508-1-AP, 4ug; Detection:10508-1-AP 1:300) with Jurkat cells lysate 2120ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human colon cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human colon cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human urothelial carcinoma using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human urothelial carcinoma using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human breast cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human breast cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human stomach cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SURVIVIN Polyclonal antibody (10508-1-AP)

    IHC staining of human stomach cancer using 10508-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SURVIVIN Polyclonal antibody (10508-1-AP)

    IF Staining of MCF-7 using 10508-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SURVIVIN antibody (10508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using SURVIVIN Polyclonal antibody (10508-1-AP)

    FC experiment of Jurkat using 10508-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug SURVIVIN Polyclonal antibody (10508-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, A549 cells, MCF-7 cells, A431 cells, Ramos cells
Positive IP detected inU2OS cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human urothelial carcinoma tissue, human stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10508-1-AP targets SURVIVIN in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SURVIVIN fusion protein Ag0788 相同性解析による交差性が予測される生物種
Full Name baculoviral IAP repeat-containing 5
Calculated molecular weight 16 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberBC008718
Gene symbol Survivin
Gene ID (NCBI) 332
RRIDAB_2064048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Protocols

Product Specific Protocols
WB protocol for SURVIVIN antibody 10508-1-APDownload protocol
IHC protocol for SURVIVIN antibody 10508-1-APDownload protocol
IF protocol for SURVIVIN antibody 10508-1-APDownload protocol
IP protocol for SURVIVIN antibody 10508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomaterials

PMID: 29028548

Authors - Yudong Song
View Article
human,mouseWB

Theranostics

Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Authors - Bojun Li
View Article
humanWB

Autophagy

Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells.

Authors - Xuejiao Zhao
View Article
humanWB

Circ Res

Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat.

Authors - Emanuele Cotroneo
View Article
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
View Article
humanWB

Cancer Lett

SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation

Authors - Jiahui Yuan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Susmita (Verified Customer) (08-21-2020)

Giving good result and crisp band in Western Blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Pancreatic cell line
SURVIVIN Antibody Western Blot validation (1:1000 dilution) in Pancreatic cell line (Cat no:10508-1-AP)
FH

Iram (Verified Customer) (08-14-2020)

We got very sharp and clean band of survivin with this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human colon cancer