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  • Featured Product
  • KD/KO Validated

SURVIVIN Monoclonal antibody, PBS Only

SURVIVIN Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B9H7

Cat no : 66495-1-PBS

Synonyms

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SURVIVIN Monoclonal antibody (66495-1-Ig)

    WB analysis using 66495-1-Ig (same clone as 66495-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using SURVIVIN Monoclonal antibody (66495-1-Ig)

    WB analysis of Raji using 66495-1-Ig (same clone as 66495-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SURVIVIN Monoclonal antibody (66495-1-Ig)

    WB analysis of K-562 using 66495-1-Ig (same clone as 66495-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SURVIVIN Monoclonal antibody (66495-1-Ig)

    IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SURVIVIN Monoclonal antibody (66495-1-Ig)

    IHC staining of human skin cancer using 66495-1-Ig (same clone as 66495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SURVIVIN Monoclonal antibody (66495-1-Ig)

    IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66495-1-PBS targets SURVIVIN in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SURVIVIN fusion protein Ag20958 相同性解析による交差性が予測される生物種
Full Name baculoviral IAP repeat-containing 5
Calculated molecular weight 16 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC008718
Gene symbol Survivin
Gene ID (NCBI) 332
RRIDAB_2881860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.