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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUSD2 Polyclonal antibody (30711-1-AP)

    WB analysis using 30711-1-AP

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 30711-1-AP (SUSD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SUSD2 Polyclonal antibody (30711-1-AP)

    WB analysis using 30711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30711-1-AP (SUSD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SUSD2 Polyclonal antibody (30711-1-AP)

    IHC staining of mouse kidney using 30711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30711-1-AP (SUSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SUSD2 Polyclonal antibody

SUSD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30711-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

sushi domain containing 2, BK65A6.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSK-BR-3 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30711-1-AP targets SUSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUSD2 fusion protein Ag33630 相同性解析による交差性が予測される生物種
Full Name sushi domain containing 2
Calculated molecular weight90 kDa
Observed molecular weight60 kDa, 90 kDa, 110 kDa
GenBank accession numberNM_019601
Gene Symbol SUSD2
Gene ID (NCBI) 56241
RRIDAB_3669753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGT4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sushi domain containing 2 (SUSD2) is a type I transmembrane protein that contains a somatomedin B, AMOP, von Willebrand factor type D and Sushi domains, which are frequently found in molecules associated with cell-cell and cell-matrix adhesion. SUSD2 is composed of 822 amino acids with a predicted molecular weight of 90.4 kDa (PMID: 31387209). Three specific SUSD2 bands were present, the glycosylated, full-length protein (~110 kDa), ~90 kDa band represents full-length SUSD2 with minimal glycosylation, and a strong ~60 kDa band, represents a cleaved product of SUSD2 at the GD-PH sequence in the endoplasmic reticulum (PMID:27775699, 32595828).

Protocols

Product Specific Protocols
WB protocol for SUSD2 antibody 30711-1-APDownload protocol
IHC protocol for SUSD2 antibody 30711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols