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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SUSD4 Polyclonal antibody

SUSD4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26829-1-AP

Synonyms

PRO222, SUSD4, sushi domain containing 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUSD4 Polyclonal antibody (26829-1-AP)

    WB analysis using 26829-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26829-1-AP (SUSD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SUSD4 Polyclonal antibody (26829-1-AP)

    IHC staining of mouse cerebellum using 26829-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26829-1-AP (SUSD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using SUSD4 Polyclonal antibody (26829-1-AP)

    IF Staining of mouse cerebellum using 26829-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SUSD4 antibody (26829-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26829-1-AP targets SUSD4 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUSD4 fusion protein Ag25346 相同性解析による交差性が予測される生物種
Full Name sushi domain containing 4
Calculated molecular weight 490 aa, 54 kDa
Observed molecular weight47 kDa
GenBank accession numberBC004888
Gene symbol SUSD4
Gene ID (NCBI) 55061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The SUSD4 (Sushi domain-containing protein 4) gene resides on chromosome 1q41 and encodes a 49-kD transmembrane protein containing four extracellular Sushi domain motifs. The Sushi domain, also known as the complement control protein domain, is commonly involved in protein-protein interactions. Many complement regulators are constructed with complement control protein domains. SUSD4 functions as a complement system regulator, as it has been shown to bind to the C1 complex and complement component C1q and block the activation of the complement cascade by interrupting the formation of C3 convertase. (PMID: 32179479)

Protocols

Product Specific Protocols
WB protocol for SUSD4 antibody 26829-1-APDownload protocol
IHC protocol for SUSD4 antibody 26829-1-APDownload protocol
IF protocol for SUSD4 antibody 26829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols