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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SWAP70 Polyclonal antibody (31042-1-AP)

    WB analysis using 31042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31042-1-AP (SWAP70 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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SWAP70 Polyclonal antibody

SWAP70 Polyclonal Antibody for WB, ELISA
Cat No. 31042-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

FLJ39540, HSPC321, KIAA0640, SWAP 70, SWAP 70 protein, SWAP70, Switch associated protein 70

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31042-1-AP targets SWAP70 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SWAP70 fusion protein Ag34855 相同性解析による交差性が予測される生物種
Full Name SWAP-70 protein
Calculated molecular weight 69 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC000616
Gene Symbol SWAP70
Gene ID (NCBI) 23075
RRIDAB_3669827
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UH65
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).

Protocols

Product Specific Protocols
WB protocol for SWAP70 antibody 31042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols