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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SWAP70 Recombinant antibody (83646-6-RR)

    WB analysis using 83646-6-RR (same clone as 83646-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83646-6-RR (SWAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SWAP70 Recombinant antibody (83646-6-RR)

    IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SWAP70 Recombinant antibody (83646-6-RR)

    IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SK-N-SH cells using SWAP70 Recombinant antibody (83646-6-RR)

    FC experiment of SK-N-SH using 83646-6-RR (same clone as 83646-6-PBS)

    1x10^6 SK-N-SH cells were intracellularly stained with 0.25 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using SWAP70 Recombinant antibody (83646-6-RR)

    FC experiment of U2OS using 83646-6-RR (same clone as 83646-6-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.2 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83646-6-RR

    Affinity and Kinetic Characterization of 83646-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83646-6-RR against Human SWAP70 were performed. The affinity constant is below 1 pM.

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SWAP70 Recombinant antibody, PBS Only

SWAP70 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83646-6-PBS
Clone No.240641F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

SWAP-70, SWAP 70 protein, SWAP 70, KIAA0640, HSPC321

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83646-6-PBS targets SWAP70 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SWAP70 fusion protein Ag34855 相同性解析による交差性が予測される生物種
Full Name SWAP-70 protein
Calculated molecular weight 69 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC000616
Gene Symbol SWAP70
Gene ID (NCBI) 23075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UH65
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).