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  • Featured Product
  • KD/KO Validated

SYAP1 Polyclonal antibody

SYAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16272-1-AP

Synonyms

PRO3113, SYAP1, Synapse associated protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYAP1 Polyclonal antibody (16272-1-AP)

    WB analysis using 16272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SYAP1 Polyclonal antibody (16272-1-AP)

    WB analysis using 16272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using SYAP1 Polyclonal antibody (16272-1-AP)

    WB analysis of SMMC-7721 using 16272-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SYAP1 Polyclonal antibody (16272-1-AP)

    WB analysis of A431 using 16272-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using SYAP1 Polyclonal antibody (16272-1-AP)

    IP experiment of L02 using 16272-1-AP

    IP result of anti-SYAP1 (IP:16272-1-AP, 4ug; Detection:16272-1-AP 1:500) with L02 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SYAP1 Polyclonal antibody (16272-1-AP)

    IHC staining of human cervical cancer using 16272-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SYAP1 Polyclonal antibody (16272-1-AP)

    IHC staining of human cervical cancer using 16272-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SYAP1 Polyclonal antibody (16272-1-AP)

    IHC staining of human small intestine using 16272-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SYAP1 Polyclonal antibody (16272-1-AP)

    IHC staining of human small intestine using 16272-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYAP1 Polyclonal antibody (16272-1-AP)

    IF Staining of HepG2 using 16272-1-AP

    Immunofluorescent analysis of HepG2 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYAP1 Polyclonal antibody (16272-1-AP)

    IF Staining of HepG2 using 16272-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (16272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SYAP1 Polyclonal antibody (16272-1-AP)

    IF Staining of A431 using 16272-1-AP

    Immunofluorescent analysis of A431 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, SMMC-7721 cells, A431 cells, mouse lung tissue, HepG2 cells, L02 cells, mouse liver tissue, rat lung tissue
Positive IHC detected inhuman cervical cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16272-1-AP targets SYAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYAP1 fusion protein Ag9342 相同性解析による交差性が予測される生物種
Full Name synapse associated protein 1, SAP47 homolog (Drosophila)
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC014657
Gene symbol SYAP1
Gene ID (NCBI) 94056
RRIDAB_2878236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

Protocols

Product Specific Protocols
WB protocol for SYAP1 antibody 16272-1-APDownload protocol
IHC protocol for SYAP1 antibody 16272-1-APDownload protocol
IF protocol for SYAP1 antibody 16272-1-APDownload protocol
IP protocol for SYAP1 antibody 16272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article
mouseWB,IF

Histochem Cell Biol

Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons.

Authors - Dominique Schmitt
  • KO Validated
View Article
mouseIF

Biol Open

An essential role of the mouse synapse-associated protein Syap1 in circuits for spontaneous motor activity and rotarod balance.

Authors - Cora R von Collenberg
View Article