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  • Featured Product
  • KD/KO Validated

SYCE1 Polyclonal antibody

SYCE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17406-1-AP

Synonyms

C10orf94, Cancer/testis antigen 76, CT76, SYCE1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SYCE1 Polyclonal antibody (17406-1-AP)

    WB analysis of mouse testis using 17406-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17406-1-AP (SYCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using SYCE1 Polyclonal antibody (17406-1-AP)

    WB analysis of rat testis using 17406-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 17406-1-AP (SYCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SYCE1 Polyclonal antibody (17406-1-AP)

    IHC staining of human testis using 17406-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SYCE1 Polyclonal antibody (17406-1-AP)

    IHC staining of human testis using 17406-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SYCE1 Polyclonal antibody (17406-1-AP)

    IHC staining of human ovary using 17406-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SYCE1 Polyclonal antibody (17406-1-AP)

    IHC staining of human ovary using 17406-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17406-1-AP targets SYCE1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYCE1 fusion protein Ag11426 相同性解析による交差性が予測される生物種
Full Name synaptonemal complex central element protein 1
Calculated molecular weight 315 aa, 36 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC034821
Gene symbol SYCE1
Gene ID (NCBI) 93426
RRIDAB_10597113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SYCE1 antibody 17406-1-APDownload protocol
IHC protocol for SYCE1 antibody 17406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Adv

Meiotic chromosome synapsis depends on multivalent SYCE1-SIX6OS1 interactions that are disrupted in cases of human infertility.

Authors - Fernando Sánchez-Sáez
  • KO Validated
View Article
humanWB,IHC

J Cell Mol Med

Novel exon mutation in SYCE1 gene is associated with non-obstructive azoospermia.

Authors - Ke Feng
View Article