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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SYCP1 Recombinant antibody (83382-1-RR)

    WB analysis of mouse testis using 83382-1-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 83382-1-RR (SYCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using SYCP1 Recombinant antibody (83382-1-RR)

    FC experiment of MCF-7 using 83382-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Sycp1 Recombinant Antibody (83382-1-RR, Clone:240033E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83382-1-RR

    Affinity and Kinetic Characterization of 83382-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83382-1-RR against Human SYCP1 were performed. The affinity constant is below 1 pM.

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SYCP1 Recombinant antibody

SYCP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83382-1-RR
Clone No.240033E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

SYCP 1, SCP-1, SCP1, SCP 1, CT8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83382-1-RR targets SYCP1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYCP1 fusion protein Ag16005 相同性解析による交差性が予測される生物種
Full Name synaptonemal complex protein 1
Calculated molecular weight 976 aa, 114 kDa
Observed molecular weight115-120 kDa
GenBank accession numberBC126266
Gene Symbol SYCP1
Gene ID (NCBI) 6847
RRIDAB_3671036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SYCP1 antibody 83382-1-RRDownload protocol
FC protocol for SYCP1 antibody 83382-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols