Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, HepG2 cells, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 3 publications below |
| IF | See 19 publications below |
Product Information
23024-1-AP targets SCP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SCP3 fusion protein Ag19330 相同性解析による交差性が予測される生物種 |
| Full Name | synaptonemal complex protein 3 |
| Calculated molecular weight | 236 aa, 28 kDa |
| Observed molecular weight | 30 kDa, 33 kDa |
| GenBank accession number | BC062662 |
| Gene Symbol | SYCP3 |
| Gene ID (NCBI) | 50511 |
| RRID | AB_11232426 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZU3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCP3 antibody 23024-1-AP | Download protocol |
| IF protocol for SCP3 antibody 23024-1-AP | Download protocol |
| IP protocol for SCP3 antibody 23024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination. | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. | ||
Int J Nanomedicine Eugenol Nanoparticles Ameliorate Doxorubicin-Induced Spermatogenic Dysfunction by Inhibiting the PINK1/Parkin and BNIP3/NIX Signaling Pathways | ||
Int J Nanomedicine Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway. | ||
Biomed Pharmacother Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway. |