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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SYCP3 Polyclonal antibody

SYCP3 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA, IF

Conjugate

Unconjugated

Cat no : 30079-1-AP

Synonyms

SCP3, Synaptonemal complex protein 3, SCP-3, SCP 3, COR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYCP3 Polyclonal antibody (30079-1-AP)

    WB analysis using 30079-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30079-1-AP (SYCP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using SYCP3 Polyclonal antibody (30079-1-AP)

    FC experiment of Jurkat using 30079-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human SYCP3 (30079-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

30079-1-AP targets SYCP3 in WB, FC (Intra), ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYCP3 fusion protein Ag32466 相同性解析による交差性が予測される生物種
Full Name synaptonemal complex protein 3
Calculated molecular weight 236 aa, 28 kDa
Observed molecular weight 27 kDa, 28 kDa
GenBank accession numberBC062662
Gene symbol SYCP3
Gene ID (NCBI) 50511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.

Protocols

Product Specific Protocols
WB protocol for SYCP3 antibody 30079-1-APDownload protocol
FC protocol for SYCP3 antibody 30079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Elife

Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual

Authors - Tianyu Zhu
View Article