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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using SCP3 Monoclonal antibody (66409-1-Ig)

    WB analysis of human testis using 66409-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66409-1-Ig (SYCP3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using SCP3 Monoclonal antibody (66409-1-Ig)

    WB analysis of DU 145 using 66409-1-Ig

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66409-1-Ig (SYCP3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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SCP3 Monoclonal antibody

SCP3 Monoclonal Antibody for WB, ELISA
Cat No. 66409-1-Ig
Clone No.1A11F1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

COR1, SCP 3, SCP3, SYCP3, synaptonemal complex protein 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, DU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66409-1-Ig targets SCP3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SCP3 fusion protein Ag19330 相同性解析による交差性が予測される生物種
Full Name synaptonemal complex protein 3
Calculated molecular weight 236 aa, 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC062662
Gene Symbol SYCP3
Gene ID (NCBI) 50511
RRIDAB_2881781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IZU3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.

Protocols

Product Specific Protocols
WB protocol for SCP3 antibody 66409-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols