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  • Featured Product
  • KD/KO Validated

SYF2 Polyclonal antibody

SYF2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Cat no : 16958-1-AP

Synonyms

Pre-mRNA-splicing factor SYF2, Pre mRNA splicing factor SYF2, GCIPIP, CCNDBP1-interactor, CCNDBP1 interactor

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SYF2 Polyclonal antibody (16958-1-AP)

    WB analysis of HEK-293 using 16958-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16958-1-AP (SYF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SYF2 Polyclonal antibody (16958-1-AP)

    WB analysis of HEK-293 using 16958-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16958-1-AP (SYF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SYF2 Polyclonal antibody (16958-1-AP)

    IF Staining of HEK-293 using 16958-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 16958-1-AP (SYF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16958-1-AP targets SYF2 in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYF2 fusion protein Ag10656 相同性解析による交差性が予測される生物種
Full Name SYF2 homolog, RNA splicing factor (S. cerevisiae)
Calculated molecular weight 243 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC010862
Gene symbol SYF2
Gene ID (NCBI) 25949
RRIDAB_2197205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SYF2 antibody 16958-1-APDownload protocol
IF protocol for SYF2 antibody 16958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,RIP

Nucleic Acids Res

ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.

Authors - Iris Tanaka
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody recognize a strong band of the correct size. Also two lower nolecular weight non-specific bands are visible

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mESCs
SYF2 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:16958-1-AP)