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  • Featured Product
  • KD/KO Validated

SYK Polyclonal antibody

SYK Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22206-1-AP

Synonyms

Tyrosine-protein kinase SYK, Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SYK Polyclonal antibody (22206-1-AP)

    WB analysis of HEK-293 using 22206-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22206-1-AP (SYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SYK Polyclonal antibody (22206-1-AP)

    WB analysis of HEK-293 using 22206-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22206-1-AP (SYK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SYK Polyclonal antibody (22206-1-AP)

    IP experiment of HEK-293 using 22206-1-AP

    IP result of anti-SYK (IP:22206-1-AP, 4ug; Detection:22206-1-AP 1:500) with HEK-293 cells lysate 2700ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using SYK Polyclonal antibody (22206-1-AP)

    IHC staining of human spleen using 22206-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using SYK Polyclonal antibody (22206-1-AP)

    IHC staining of human spleen using 22206-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of Ramos cells using SYK Polyclonal antibody (22206-1-AP)

    FC experiment of Ramos using 22206-1-AP

    1x10^6 Ramos cells were intracellularly stained with 0.4 ug SYK Polyclonal antibody (22206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22206-1-AP targets SYK in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYK fusion protein Ag17526 相同性解析による交差性が予測される生物種
Full Name spleen tyrosine kinase
Calculated molecular weight 635 aa, 72 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC001645
Gene symbol SYK
Gene ID (NCBI) 6850
RRIDAB_2879028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

Protocols

Product Specific Protocols
WB protocol for SYK antibody 22206-1-APDownload protocol
IHC protocol for SYK antibody 22206-1-APDownload protocol
IP protocol for SYK antibody 22206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virulence

Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK.

Authors - Xuelin Han
View Article
mouseWB

Biochim Biophys Acta

Syk negatively regulates TLR4-mediated IFNβ and IL-10 production and promotes inflammatory responses in dendritic cells.

Authors - Hui Yin
  • KO Validated
View Article