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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-SYK (Tyr323) Recombinant antibody (83331-1-RR)

    WB analysis using 83331-1-RR (same clone as 83331-1-PBS)

    Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using Phospho-SYK (Tyr323) Recombinant antibody (83331-1-RR)

    IF Staining of Ramos using 83331-1-RR (same clone as 83331-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.

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Phospho-SYK (Tyr323) Recombinant antibody, PBS Only

Phospho-SYK (Tyr323) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 83331-1-PBS
Clone No.2H24

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SYK, spleen tyrosine kinase, p72-Syk, IMD82, EC:2.7.10.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83331-1-PBS targets Phospho-SYK (Tyr323) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name spleen tyrosine kinase
Calculated molecular weight 72 kDa
GenBank accession numberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP43405
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.