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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SYN1-Specific Polyclonal antibody (20258-1-AP)

    WB analysis of mouse brain using 20258-1-AP (same clone as 20258-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20258-1-AP (SYN1-Specific Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20258-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using SYN1-Specific Polyclonal antibody (20258-1-AP)

    IP experiment of rat brain using 20258-1-AP (same clone as 20258-1-PBS)

    IP result of anti-SYN1-Specific (IP:20258-1-AP, 4ug; Detection:20258-1-AP 1:3000) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 20258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SYN1-Specific Polyclonal antibody (20258-1-AP)

    IHC staining of mouse brain using 20258-1-AP (same clone as 20258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SYN1-Specific Polyclonal antibody (20258-1-AP)

    IHC staining of mouse brain using 20258-1-AP (same clone as 20258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20258-1-PBS in a different storage buffer formulation.

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SYN1-Specific Polyclonal antibody, PBS Only

SYN1-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20258-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Synapsin I, SYN1, Brain protein 4.1, Synapsin 1, SYN1b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20258-1-PBS targets SYN1-Specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synapsin I
Calculated molecular weight 74 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberNM_006950
Gene Symbol SYN1
Gene ID (NCBI) 6853
RRIDAB_2800493
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17600
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SYN1, also named Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).