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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYN1-Specific Recombinant antibody (85360-1-RR)

    WB analysis using 85360-1-RR (same clone as 85360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85360-1-RR (SYN1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse retina tissue using SYN1-Specific Recombinant antibody (85360-1-RR)

    IF Staining of mouse retina using 85360-1-RR (same clone as 85360-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using SYN1-Specific antibody (85360-1-RR, Clone: 242415H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85360-1-PBS in a different storage buffer formulation.

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SYN1-Specific Recombinant antibody, PBS Only

SYN1-Specific Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 85360-1-PBS
Clone No.242415H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

SYN1, Synapsin I, 242415H5, Brain protein 4.1, SYN1a

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85360-1-PBS targets SYN1-Specific in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synapsin I
Calculated molecular weight 74 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberNM_006950
Gene Symbol SYN1
Gene ID (NCBI) 6853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17600
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SYN1, also named Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).