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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nesprin 2 Polyclonal antibody

Nesprin 2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25265-1-AP

Synonyms

SYNE2, SYNE 2, NUANCE, NUA

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Nesprin 2 Polyclonal antibody (25265-1-AP)

    WB analysis of HEK-293 using 25265-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25265-1-AP (SYNE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of mouse brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of mouse brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of rat brain using 25265-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Human dorsal cerebral organoid using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of Human dorsal cerebral organoid using 25265-1-AP

    Human dorsal cerebral organoid at 12 weeks. 4% PFA fixed. Antibody dilution was 1:500 for Syne-2 (25265-1-AP; white), DAPI 1:1000. Image created by Wimmer Ryszard from the Institut Curie in Paris, France

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IF detected inHuman dorsal cerebral organoid, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25265-1-AP targets Nesprin 2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name spectrin repeat containing, nuclear envelope 2
Calculated molecular weight 799 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberNM_015180
Gene symbol Nesprin 2
Gene ID (NCBI) 23224
RRIDAB_2879995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.

Protocols

Product Specific Protocols
WB protocol for Nesprin 2 antibody 25265-1-APDownload protocol
IF protocol for Nesprin 2 antibody 25265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer

Authors - Bei-Bei Sun
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ryszard (Verified Customer) (07-25-2022)

Very nice staining with the Syne-2 antibody. I tried on Human Fetal Brain Tissue, Human Cerebral Organoids and Human Neural Stem Cells. They are very nice. Images attached to this comment from a Human Cerebral Organoid (Staining of Nesprin-2 in white LUT)

  • Applications: Immunohistochemistry, Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Cerebral Organoids/Human Fetal Brain Tissue and Neural Stem Cells
Nesprin 2 Antibody Immunohistochemistry,Immunofluorescence,Cell culture validation (1:500 dilution) in Human Cerebral Organoids/Human Fetal Brain Tissue and Neural Stem Cells (Cat no:25265-1-AP)