Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85045-1-RR targets SYNGAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | synaptic Ras GTPase activating protein 1 homolog (rat) |
| Calculated molecular weight | 1343 aa, 148 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_006772 |
| Gene Symbol | SYNGAP1 |
| Gene ID (NCBI) | 8831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q96PV0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SYNGAP1 antibody 85045-1-RR | Download protocol |
| IF protocol for SYNGAP1 antibody 85045-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |