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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNGR1 Recombinant antibody (84161-3-RR)

    WB analysis using 84161-3-RR (same clone as 84161-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84161-3-RR (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84161-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SYNGR1 Recombinant antibody (84161-3-RR)

    WB analysis of pig brain using 84161-3-RR (same clone as 84161-3-PBS)

    pig brain tissue was subjected to SDS PAGE followed by western blot with 84161-3-RR (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84161-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SYNGR1 Recombinant antibody (84161-3-RR)

    IF Staining of mouse brain using 84161-3-RR (same clone as 84161-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNGR1 antibody (84161-3-RR, Clone: 241460D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84161-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84161-3-RR

    Affinity and Kinetic Characterization of 84161-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84161-3-RR against Human SYNGR1 were performed. The affinity constant is 3.48 nM.

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SYNGR1 Recombinant antibody, PBS Only

SYNGR1 Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 84161-3-PBS
Clone No.241460D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, Indirect ELISA

Synonyms

Synaptogyrin-1, synaptogyrin 1, 241460D1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84161-3-PBS targets SYNGR1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYNGR1 fusion protein Ag0685 相同性解析による交差性が予測される生物種
Full Name synaptogyrin 1
Calculated molecular weight 25 kDa
Observed molecular weight30 kDa
GenBank accession numberBC000731
Gene Symbol SYNGR1
Gene ID (NCBI) 9145
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43759
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)