Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, rat brain tissue |
| Positive IHC detected in | human brain tissue, human skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
24677-1-AP targets SYNJ1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SYNJ1 fusion protein Ag20364 相同性解析による交差性が予測される生物種 |
| Full Name | synaptojanin 1 |
| Calculated molecular weight | 1612 aa, 178 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC136603 |
| Gene Symbol | SYNJ1 |
| Gene ID (NCBI) | 8867 |
| RRID | AB_2879668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43426 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues.(PMID:10801423).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SYNJ1 antibody 24677-1-AP | Download protocol |
| IHC protocol for SYNJ1 antibody 24677-1-AP | Download protocol |
| IF protocol for SYNJ1 antibody 24677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Chem Biol Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome. | ||
Behav Brain Res SYNJ1 rescues motor functions in hereditary and sporadic Parkinson's disease mice by upregulating TSP-1 expression | ||
Ren Fail Network pharmacology and molecular docking of endogenous active metabolites in diabetic kidney disease | ||
Cell Signal Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen. |