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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNJ2 Polyclonal antibody (30391-1-AP)

    WB analysis using 30391-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SYNJ2 Polyclonal antibody (30391-1-AP)

    WB analysis using 30391-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SYNJ2 Polyclonal antibody (30391-1-AP)

    IHC staining of mouse small intestine using 30391-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SYNJ2 Polyclonal antibody (30391-1-AP)

    IHC staining of mouse small intestine using 30391-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SYNJ2 Polyclonal antibody (30391-1-AP)

    IF Staining of U-251 using 30391-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using SYNJ2 antibody (30391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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SYNJ2 Polyclonal antibody

SYNJ2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30391-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

synaptojanin 2, Synaptojanin-2, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2, KIAA0348, INPP5H

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30391-1-AP targets SYNJ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYNJ2 fusion protein Ag33002 相同性解析による交差性が予測される生物種
Full Name synaptojanin 2
Calculated molecular weight 1496 aa, 166 kDa
Observed molecular weight143 kDa, 160 kDa
GenBank accession numberBC043277
Gene Symbol SYNJ2
Gene ID (NCBI) 8871
RRIDAB_3086305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15056
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SYNJ2 antibody 30391-1-APDownload protocol
IHC protocol for SYNJ2 antibody 30391-1-APDownload protocol
IF protocol for SYNJ2 antibody 30391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols