Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83532-3-RR targets SYNPO2L in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SYNPO2L fusion protein Ag34027 相同性解析による交差性が予測される生物種 |
| Full Name | synaptopodin 2-like |
| Calculated molecular weight | 977 aa, 102 kDa |
| GenBank accession number | BC142635 |
| Gene Symbol | SYNPO2L |
| Gene ID (NCBI) | 79933 |
| RRID | AB_3671157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H987 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SYNPO2L antibody 83532-3-RR | Download protocol |
| FC protocol for SYNPO2L antibody 83532-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |